A Novel Dialkylamino GFP Chromophore as an Environment-Polarity Sensor Reveals the Role of Twisted Intramolecular Charge Transfer
نویسندگان
چکیده
We discovered a novel fluorophore by incorporating dimethylamino group (–NMe2) into the conformationally locked green fluorescent protein (GFP) scaffold. It exhibited marked solvent-polarity-dependent fluorogenic behavior and can potentially find broad applications as an environment-polarity sensor in vitro vivo. The ultrafast femtosecond transient absorption (fs-TA) spectroscopy combination with quantum calculations revealed presence of twisted intramolecular charge transfer (TICT) state, which is formed rotation –NMe2 electronic excited state. In contrast to bright state (FS), TICT dark effectively quenches fluorescence upon formation. employed newly developed multivariable analysis approach FS lifetime various solvents showed that ? reaction barrier mainly modulated H-bonding capability instead viscosity solvent, accounting for observed polarity dependence. These deep mechanistic insights are further corroborated dramatic loss fluorogenicity two similar GFP-derived chromophores inhibited structural locking.
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ژورنال
عنوان ژورنال: Chemosensors
سال: 2021
ISSN: ['2227-9040']
DOI: https://doi.org/10.3390/chemosensors9080234